Deciphering cell signaling networks through innovative proteomics.
Global mapping of the endogenous DNA damage repair networks identifies Shieldin as a novel protein complex in DNA damage repair.
Gupta R et al. Cell. 2018 May 3.[Pubmed]
Using a novel proteomic approach we reveal a comprehensive map of protein interaction of key DNA damage repair proteins. We show that the novel protein complex shieldin is an essential downstream effector of 53BP1 in regulating NHEJ, antibody class-switching and sensitivity to PARP inhibitors. Identification of Shieldin provides new insights into the evolution of vertebrate-specific DDR pathways with key relevance for understanding antibody diversification and PARP inhibitor resistance.
Time-resolved analysis reveals rapid dynamics and broad Scope of the CBP/p300 acetylome.
Weinert BT, Narita T et al. Cell. 2018 May 14. [Pubmed]
By combining quantitative proteomics with specific CBP/p300 catalytic inhibitors, bromodomain inhibitors, and genetic knockout cells, we reveal a comprehensive map of regulated acetylation sites and their dynamic turnover rates. CBP/p300 regulates a much larger number of acetylation sites than known previously, and CBP/p300-catalyzed rapid acetylation is essential for gene transcription. The entire resource dataset is freely accessible at:p300db.choudharylab.org
A fully-funded postdoc position is available in the Group.
We offer exciting scientific environment and cutting-edge technologies for identifying and investigating novel regulatory mechanisms of cell signaling. More details about the position can be found here.
Interested candidates can also directly contact Chuna Choudhary:email@example.com